What is the optimal light source for optical mapping using voltage- and calcium-sensitive dyes?
نویسندگان
چکیده
منابع مشابه
Use of Voltage - Sensitive Dyes and Optical
-Understanding the spatio-temporal features of the information processing occurring in any complex neural structure requires the monitoring and analysis of the activity in populations of neurons. Electrophysiological and other mapping techniques have provided important insights into the function of neural circuits and neural populations in many systems. However, there remain limitations with th...
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lonophore-based ion-selective optical nanosensors have been explored for a number of years. Voltage sensitive dyes (VSDs) have been introduced into this type of sensors only very recently, forming a new class of analytical tools. Here, K(+)-sensitive nanospheres incorporating a lipophilic VSD were successfully fabricated and characterized. The nanosensors were readily delivered into the social ...
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We carried out experiments to monitor optically the generation and spread of action potentials and subthreshold potentials in the processes of individual neurons in ganglia of the snail, Helix aspersa. The neurons were selectively stained by intracellular pressure injection of voltage-sensitive dyes. Optical signals were detected by a system for fast, multiple-site optical monitoring, utilizing...
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We describe the characteristics of low-cost ultra-high-power light emitting diodes (LEDs) for use in optical imaging experiments. We use the LEDs in experiments with bullfrog cardiac tissue and find that the signal-to-noise ratio is comparable to other commonly used illumination sources. Contact Information: Duke University, Department of Physics, Box 90305, Durham, NC, 27708 Tel: (919) 660-251...
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ژورنال
عنوان ژورنال: Physiological Research
سال: 2020
ISSN: 1802-9973,0862-8408
DOI: 10.33549/physiolres.934471